Coding

Part:BBa_K3468031:Design

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-19)


PETase V68I


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. V68I was designed by searching for suitable sites around Y70 for mutation.


Source

Ideonalla sakaiensis

References